Journal: Clinical Chemistry

Article Title: Immunological measurement of transforming growth factor-beta 1 (TGF-β1) in blood; assay development and comparison

doi: 10.1093/clinchem/43.10.1965

Figure Lengend Snippet: Fig. 1. Calibration curve and im- precision at each calibrator con- centration (median values of n 5 13 runs) for the in-house TGF-b1 immunoassay.

Article Snippet: Commercial immunoassay kits for the comparison determinations of TGF-b1 were purchased: Predicta (Genzyme), Quantikine (R&D Systems, Minneapolis, MN), Amersham (Amersham International), and Promega (Promega).

Techniques:

Journal: Clinical Chemistry

Article Title: Immunological measurement of transforming growth factor-beta 1 (TGF-β1) in blood; assay development and comparison

doi: 10.1093/clinchem/43.10.1965

Figure Lengend Snippet: Fig. 2. Effect of serial sample dilution on the apparent concentrations measured by 4 commer- cially available TGF-b1 immunoassays performed with the activation procedures recommended by the manufacturers.

Article Snippet: Commercial immunoassay kits for the comparison determinations of TGF-b1 were purchased: Predicta (Genzyme), Quantikine (R&D Systems, Minneapolis, MN), Amersham (Amersham International), and Promega (Promega).

Techniques: Activation Assay

Journal: Endocrinology

Article Title: Function of Stem Cell Factor as a Survival Factor of Spermatogonia and Localization of Messenger Ribonucleic Acid in the Rat Seminiferous Epithelium

doi: 10.1210/en.140.3.1492

Figure Lengend Snippet: FIG. 4. The [3H]thymidine incorporation (counts per min; mean 6 SEM; n 5 12; normalized to 2 mm of tubule) at stages I, V, VIIa, VIII–IX, and XII of seminiferous epithelium cultured for 24 (A), 48 (B), and 72 h (C) with 0, 20, and 100 ng/ml mouse recombinant SCF. No significant effects on stage-specific DNA synthesis could be detected at any time point studied at stages V, VIIa, and I of the cycle. SCF

Article Snippet: Recombinant mouse SCF (Genzyme Transgenics Corp., Cambridge, MA) was added at concentrations of 0, 20, and 100 ng/ml.

Techniques: Cell Culture, Recombinant, DNA Synthesis

Journal: Endocrinology

Article Title: Function of Stem Cell Factor as a Survival Factor of Spermatogonia and Localization of Messenger Ribonucleic Acid in the Rat Seminiferous Epithelium

doi: 10.1210/en.140.3.1492

Figure Lengend Snippet: FIG. 5. Autoradiography of the seminiferous tubules from stage XII of the seminiferous epithelial cycle cultured for 72 h in the absence (A) and presence (B) of recombinant human SCF (100 ng/ml). The tubule segments from stage XII of the cycle proceed to stage I of the cycle during the culture. Spermatogonia in the control tubules show minimal grain accumulation (A), whereas spermatogonia in the tu- bule segments cultured in the presence of 100 ng/ml SCF show abun- dant grain accumulation. C, Quantification of the labeled cells in the 2-mm tubule segment from stage XII cultured for 72 h in the absence and presence of SCF (100 ng/ml). The control tubule segment shows 100 times fewer labeled germ cells compared with the SCF-stimulated tubule segment. Bar, 25 mm; n 5 4.

Article Snippet: Recombinant mouse SCF (Genzyme Transgenics Corp., Cambridge, MA) was added at concentrations of 0, 20, and 100 ng/ml.

Techniques: Autoradiography, Cell Culture, Recombinant, Control, Labeling